She told the audience her adopted son David, who was born in the African nation of Malawi, had lost family members to the disease.
'I should acknowledge not only the dearest and most amazing friends of mine who died of AIDS so many years ago, and who still do, but the....family of my adopted son who died of AIDS,' Madonna told the crowd. 'Let's give a big thanks to people who continue to fight to find a cure for this disease and let's acknowledge all the people who have passed who have fought to raise awareness. We shall overcome one day. Just to say thank you I'd like to sing a little prayer to you.'
Madonna has adopted two children, David and Mercy, from the impoverished country, where around 10 per cent of the population is infected with the HIV virus, which causes AIDS.
The singer has been a long-time campaigner for AIDS awareness, having lost her dance teacher and mentor Christopher Flynn to the disease in 1990.