The highest lot was a jacket from Desperately Seeking Susan, which fetched $252,000, while a gown from her Material Girl video reached $73,125.
The Californian auction also saw lots from Michael Jackson, Cher and The Beatles.
The wedding dress Madonna wore when she wed actor Sean Penn in 1985 sold for $81,250 (£51,192), while a dress she wore on her Who's That Girl tour reached bids of $50,000 (£31,503).
Other lots which attracted the bidders were a pair of John Lennon's spectacles which sold for $25,000 (£15,751) and a ring worn by Elvis Presley for $57,600 (£36,291).
Guitars from a host of rock greats formed part of the two-day sale including Prince's Sign of the Times Love guitar and a 1940 Gibson J200 owned by Stephen Stills of Crosby, Stills, Nash and Young, which sold for $43,750 (£27,565).
From BBC News
madonnalicious reader Peter visited the auction house last week and shares these pictures of some of the items that were up for sale.