The 'Hung Up' singer is set to join the rap duo on stage at the Staples Centre in Los Angeles at the 56th annual ceremony on Sunday (26.01.14) for their hit 'Same Love.'
A source told UsMagazine.com that the 55-year-old star is looking forward to collaborating with 30-year-old Ben Haggerty, better known as Macklemore, and his producer Ryan, 25, on the track, which is an anthem for same sex marriage and gay rights.
The Seattle, Washington natives have had a very successful year and are nominated for seven awards, including Album of the Year for 'The Heist,' Best New Artist and Song of the Year for 'Same Love.'
Madonna - who has previously won seven Grammy awards - last performed at the annual event in 2006.
The 56th annual Grammy Awards will be hosted by LL Cool J and broadcast live on the CBS network in the US from the Staples Centre in Los Angeles.