Popeater confirmed that the New York-themed finale will feature a mash-up of the Madonna track 'I Love New York' and 'New York, New York' from the Broadway musical On the Town.
Glee previously covered a number of Madonna songs, including 'Vogue' and 'Express Yourself' in the season one episode 'The Power of Madonna'.
The season finale sees the New Directions heading to the Big Apple to compete at the National Show Choir competition, and will also feature performances of two original songs.
Matthew Morrison, who plays Glee club coach Will Schuester, will perform his new single 'Still Got Tonight' in the episode.
The second season finale of Glee is scheduled to air on May 24.
Listen to the Glee mash-up of 'I Love New York/New York, New York' below:
From Digital Spy